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TEST METHOD
Quantitative Carrier Test for the Evaluation of Bactericidal Activity of Teat Disinfectants used in the Veterinary Area
EN 17422 is the European Standard used to evaluate the bactericidal activity of teat disinfectants specifically used in the veterinary area.
The infographic below provides a brief overview of the EN 17422 test method.
Understanding EN 17422 Testing
Application areas
This test method is designed specifically for teat disinfectants used without mechanical action for pre- or post- milking disinfection.
If your product is a surface disinfectant, EN 14349 would be suitable.
Test Protocol
Step 1: The mixture of test suspension and interfering substance is added onto the in vitro skin for conditioning of the test surface.
Step 2: The mixture is maintained on the in vitro skin for 15 minutes of conditioning time.
Step 3: 10 mL of the test product is added to immerse the inoculated in vitro skin. After 30 seconds, the in vitro skin is removed from the product and left until the end of the contact time.
Step 4: After the contact time, the in vitro skin is transferred into a neutralizer and treated in an ultrasonic bath.
Step 5: After the neutralization time, 1 mL of the mixture is plated and incubated. The surviving colonies are then enumerated.
Test Microorganisms
These are the mandatory test organisms:
- Escherichia coli
- Staphylococcus aureus
Wish to add more microorganisms? Click here for our full list of microorganisms.
Contact Time & Concentration
The test concentration is chosen according to the manufacturer’s recommendation.
The recommended contact time to be tested may be chosen from between
- Pre-milking disinfectants: 30s to 180s
- Post-milking disinfectants: 1 min to 5 mins
The obligatory test temperature by the test method is 30°C.
Soiling Conditions
Interfering substances, or commonly referred to as soiling, are used to mimic organic substances found on human skin, surfaces, and instruments. The soiling conditions for this test method are fixed as follows according to the product application:
- Pre-milking teat disinfectants: 3.0 g/L bovine serum albumin
- Post-milking teat disinfectants: 10.0 g/L milk powder
Need help with your testing requirements?
Have questions about this test method or your product? Our technical team is here to assist you.