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TEST METHOD
Quantitative Carrier Test Evaluating Virucidal Activity of Chemical Disinfectants on Non-porous Surfaces
EN 16777 is an essential European Standard used to evaluate the minimum virucidal activity requirements of surface disinfectants used on non-porous surfaces in the medical area.
The infographic below provides a brief overview of the EN 16777 test method.
Understanding EN 16777 Testing
Application Areas
This test is suitable for evaluating surface disinfectants intended for use in medical areas that do not require a wiping action.
Test Protocol
Step 1: The test suspension is inoculated onto a sterile metal disc and allowed to dry.
Step 2: The customer’s product is applied to the inoculated metal disc.
Step 3: After the specified contact time, the metal disc is transferred into an ice-cold medium and vortexed to recover the virus.
Step 4: The recovered suspension in Container B is serially diluted, and each dilution is added to a 96-well plate.
Step 5: Cell culture medium is added to the wells, and the cells are observed for the presence or absence of cytopathic effects (CPE).
Test Microorganisms
These are the mandatory virus strains for testing according to virucidal activity claims:
Virucidal Activity:
- Adenovirus
- Murine norovirus
Δ The product shall pass against Poliovirus, Adenovirus and Murine Norovirus in EN 14476 prior to EN 16777
Limited Spectrum Virucidal Activity:
- Adenovirus
- Murine norovirus
Δ The product shall pass against Adenovirus and Murine Norovirus in EN 14476 prior to EN 16777
Virucidal Activity Against Enveloped Virus:
- Vaccinia virus
Δ The product shall pass against Vaccinia virus in EN 14476 prior to EN 16777
Wish to add more microorganisms? Click here for our full list of microorganisms.
Contact Time & Concentration
The test concentration is chosen according to the manufacturer’s recommendation.
The contact time recommended by the test method is no longer than 5 minutes for surfaces frequently touched by different people, and no longer than 60 minutes for other surfaces.
Soiling Conditions
Interfering substances, or commonly referred to as soiling, are used to mimic organic substances found on human skin, surfaces, and instruments. These are the available soiling conditions according to the test method:
- Clean condition: 0.3 g/L bovine serum albumin
- Dirty condition: 3.0 g/L bovine serum albumin + 3.0 mL/L sheep erythrocytes
Need help with your testing requirements?
Have questions about this test method or your product? Our technical team is here to assist you.