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TEST METHOD
Quantitative Suspension Test for the Evaluation of Mycobactericidal Activity of Chemical Disinfectants in the Veterinary Area
EN 14204 is an European Standard used to evaluate the minimum mycobactericidal activity requirements of chemical disinfectants used in the veterinary area.
The infographic below provides a brief overview of the EN 14204 test method.
Understanding EN 14204 Testing
Test Protocol
Step 1: Customer product is added to a mixture of the test suspension and interfering substance.
Step 2: After the selected contact time, 1 mL of the test mixture is transferred into a neutralizer containing distilled water to stop the disinfectant activity.
Step 3: After the required neutralization time, the neutralized mixture is plated and incubated. Surviving bacterial colonies are then enumerated to determine the log reduction.
The test protocol is performed under the specified conditions according to the product category and intended application.
Test Microorganisms
The obligatory test organism is Mycobacterium avium.
Wish to add more microorganisms? Click here for our full list of microorganisms.
Contact Time & Concentration
The test concentration is chosen according to the manufacturer’s recommendation.
The recommended contact time chosen should be between 1 minute and 120 minutes.
The recommended test temperature by the test method is between 5°C and 40°C.
Soiling Conditions
Interfering substances, or commonly referred to as soiling, are used to mimic organic substances found on human skin, surfaces, and instruments. These are the available soiling conditions according to the test method:
- Low level soiling: 3.0 g/L bovine serum albumin
- High level soiling: 10.0 g/L bovine serum albumin + 10.0 g/L yeast extract
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