The Neat Protocol MTT ET50 Assay is an advanced in vitro method for assessing eye irritation potential without the need for animal testing. This assay utilizes the EpiOcular model, a Reconstructed Human Cornea-Like Epithelium (RhCE), to evaluate ocular irritation based on exposure time and cell viability reduction to 50% (ET50 values). The ET50 value (in minutes or hours) determines the eye irritation potential and can also be used for EPA hazard classification.
The diagram below provides a summary of the test method.