TEST METHODEN 1656

Quantitative Suspension Test for the Evaluation of Bactericidal Activity in the Veterinary Area
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EN 1656 is an essential European Standard used to evaluate the minimum bactericidal activity for chemical disinfectants used in the veterinary area. The infographic below shows a brief insight of EN 1656.
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CUSTOMER PROCESS
Step 1: Which category does your product fall under?

Is your product categorized for one of the following:

  • General disinfection
  • Teat disinfection
Step 2: Select your test microorganisms

These are the obligatory test organisms for bactericidal activity.

For general disinfection:

  • Pseudomonas aeruginosa
  • Staphylococcus aureus
  • Enterococcus hirae
  • Proteus hauseri

For teat disinfection:

  • Escherichia coli
  • Staphylococcus aureus
  • Streptococcus uberis

Wish to add more microorganisms? Click here for our full list of microorganisms.

Step 3: Select the appropriate test conditions for your test product

The test concentration is chosen according to the manufacturer’s recommendation.

The contact time recommended by the test method differs based on product application or can be chosen based on manufacturer’s recommendation.

  • The contact time recommended by the test method for general disinfection is between 1 minute to 2 hours.
  • The contact time recommended by the test method for teat disinfection is between 1 minute to 30 minutes (post-milking) or between 30 seconds to 3 minutes (pre-milking).

The test temperature recommended by the test method is between  5°C to 40°C for general disinfection or 20°C to 30°C for teat disinfection.

Step 4: What are the soiling conditions associated with your test product?

Interfering substances, or commonly referred to as soiling, are used to mimic organic substances found on human skin, surfaces, and instruments. These are the available soiling conditions according to the test method.

For general disinfection:

  • Low level soiling: 3.0 g/L bovine serum albumin
  • High level soiling: 10.0 g/L yeast extract + 10.0 g/L bovine serum albumin

For teat disinfection:

  • Post-milking: 10.0 g/L milk powder
  • Pre-milking: 3.0 g/L bovine serum albumin
Step 5: Place an order and send your sample to us

Fill in the test request form and place an order via email at info@tecolab-global.com. We will advise on the quantity of sample required for testing.

You can deliver the sample to us via courier and provide us the tracking code. We will monitor the delivery status and inform you when we receive the sample.

Step 6: Track the status of your request

Once we have received your sample, we will provide a tracking number to you. You can easily track the status of your request here.

Step 7: Download your password-protected test report

Download your readily available test report online with just a single click using the link and password provided via email notification. We will receive a notification once you have downloaded the test report.

For safety purposes, the uploaded test report will self-destruct after 7 days.

Step 8: Analyze your passing criteria in the test report with our laboratory experts

In order to pass the test, the product shall demonstrate at least a 5 decimal log (lg) reduction (99.999%).

Need assistance with your test report? Discuss your results with our microbiologists for more information.