TEST METHODEN 13727

Quantitative Suspension Test for the Evaluation of Bactericidal Activity in the Medical Area
bt_bb_section_bottom_section_coverage_image

EN 13727 is an essential European Standard used to evaluate the minimum bactericidal activity requirements of various types of products used in the medical area. The infographic below shows a brief insight of EN 13727.

Infographic of EN 13727 - Quantitative suspension test for the evaluation of bactericidal activity in the medical area.
CUSTOMER PROCESS
Step 1: Which category does your product fall under?

These are the categories of test products:

  • Hygienic handrub and handwash
  • Surgical handrub and handwash
  • Instrument disinfectant
  • Surface disinfectant
Step 2: Select your test microorganisms

These are the minimum test organisms:

  • Pseudomonas aeruginosa
  • Staphylococcus aureus
  • Enterococcus hirae

For handrub or handwash products:

  • As above, and additionally Escherichia coli K12

For instrument disinfectants used at ≥40°C:

  • Enterococcus faecium 

Wish to add more microorganisms? Click here for our full list of microorganisms.

Step 3: Select an appropriate contact time and concentration for your test product

The test concentration is chosen according to the manufacturer’s recommendation.

These are the minimum contact times given by the test method:

  • Hygienic handrub and handwash : Between 30 to 60 seconds
  • Surgical handrub and handwash : Between 1 to 5 minutes
  • Instrument disinfectant : No longer than 60 minutes
  • Surface disinfectant : No longer than 5 minutes for surfaces frequently touched by different people, and no longer than 60 minutes for other surfaces
Step 4: What are the soiling conditions associated with your test product?

Interfering substances, or commonly referred to as soiling, are used to mimic organic substances found on human skin, surfaces, and instruments. These are the available soiling conditions according to the test method:

  • Clean condition: 0.3 g/L bovine serum albumin
  • Dirty condition: 3.0 g/L bovine serum albumin + 3.0 mL/L sheep erythrocytes

These are the minimum soiling conditions for the respective categories of products:

  • Hygienic handrub : Clean condition
  • Hygienic handwash : Dirty condition
  • Surgical handrub : Clean condition
  • Surgical handwash : Dirty condition
  • Surface disinfectant : Clean or dirty condition
  • Instrument disinfectant : Clean or dirty condition
Step 5: Place an order and send your sample to us

Fill in the test request form and place an order via email at info@tecolab-global.com. We will advise on the quantity of sample required for testing.

You can deliver the sample to us via courier and provide us the tracking code. We will monitor the delivery status and inform you when we receive the sample.

Step 6: Track the status of your request

Once we have received your sample, we will provide a tracking number to you. You can easily track the status of your request here.

Step 7: Download your password-protected test report

Download your readily available test report online with just a single click using the link and password provided via email notification. We will receive a notification once you have downloaded the test report.

For safety purposes, the uploaded test report will self-destruct after 7 days.

Step 8: Analyze your passing criteria in the test report with our laboratory experts

In order to pass the test, the product shall demonstrate at least a 5 decimal log (lg) reduction (for hygienic hand wash at least a 3 lg reduction).

Need assistance with your test report? Discuss your results with our microbiologists for more information.