OECD 492 is specifically designed for hazard identification of eye irritant chemicals, biocides, and cosmetics.

If your product is a medical device, eye irritation testing may still be required, but there is no dedicated ISO 10993 standard for in vitro eye irritation testing. Thus, this method may be used to screen for the eye irritation potential of medical devices, depending on the specific regulation.

The test substance is applied directly onto the Reconstructed Human Cornea-Like Epithelium (RhCE) for

• Liquid samples: 30 minutes at 37°C
• Solid samples: 6 hours at 37°C

If cell viability after post-exposure incubation is ≤ 60%, the tested product is classified as Irritant

If cell viability after post-exposure incubation is > 60%, the tested product is classified as Non-Irritant.

*Irritant products may require further testing for classification of hazard depending on specific regulations.

If the product is classified as Non-Irritant, it is not assigned a GHS category.

If the product is classified as Irritant, further testing may be required to differentiate between GHS Category 1 (Serious Eye Damage) and GHS Category 2 (Eye Irritation).

For irritant products, the GHS hazard classification can be further predicted using a modified protocol or the EPA Eye Irritation Hazard classification with the MTT ET50 Assay.

Fill in the test request form and place an order via email at info@tecolab-global.com. We will advise on the quantity of sample required for testing.

You can deliver the sample to us via courier or utilize our sample delivery service. We will monitor the delivery status and inform you when we receive the sample.

Once we have received your sample, we will provide a tracking number to you. You can easily track the status of your request here.

Download your readily available test report online with just a single click using the link and password provided via email notification. We will receive a notification once you have downloaded the test report.

For safety purposes, the uploaded test report will self-destruct after 7 days.