TEST METHODIn-House Method 012

Evaluation of Bacterial Transfer on the Surface of Medical Examination Gloves
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In-House Method 012 is specifically designed by TECOLAB based on ASTM D7907 to evaluate the efficacy of medical examination gloves against bacteria through a dry transfer method.

The only standard method currently available to evaluate medical examination gloves against bacteria is the ASTM D7907. In this standard method that is widely practiced, bacteria is inoculated directly onto the glove surface through what is called the “wet transfer method”. Through this, the efficacy of the glove is evaluated against a wet inoculum. However, when considering real life situations in the medical area, the possibility of bacteria transfer may occur in more ways than one. Bacteria is not only present ‘wet’, but they can also dry on various surfaces of which will be frequently touched by medical professionals.

Therefore, in order to assess this alternative scenario, TECOLAB has specifically designed an in-house method based on the standard ASTM D7907 with some modifications. Instead of inoculating the gloves with bacteria directly, this method involves inoculation through what is called the “dry transfer method”, by allowing bacteria to dry on a metal carrier surface and then pressing the glove onto the carrier, mimicking the real-life action.

Through this in-house method, we can determine one of the most important infection control aspect in healthcare facilities which is evaluating the possibility of bacteria transmission between surfaces when touched by the glove.

The infographic below shows a brief insight on how we perform this in-house method.

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CUSTOMER PROCESS
Step 1: Prepare test sample and control sample

Please prepare 5 pieces of test gloves (with antimicrobial coating) and control gloves (without antimicrobial coating).

Step 2: Select your test microorganisms

There are no mandatory bacteria strains for this test. However, some of the common bacteria to start with have been:

  • Staphylococcus aureus
  • Pseudomonas aeruginosa 

Wish to add more microorganisms? Click here for our full list of microorganisms.

Step 3: Select an appropriate contact time for your test product

There is no fixed contact time for this test. It may be chosen according to manufacturer’s instructions. Common contact times chosen are 0, 5, 10, 20, and 30 minutes.

Step 4: What are the soiling conditions associated with your test product?

Interfering substances, or commonly referred to as soiling, are used to simulate the real-life usage condition of your gloves. These are the available soiling conditions according to the test method:

  • Test method A: To simulate conditions of glove without contamination by organic loading.
  • Test method B: To simulate conditions of glove contamination by organic substances expected in a healthcare environment. Test surfaces are first exposed to organic loading.
Step 5: Place an order and send your sample to us

Fill in the test request form and place an order via email at info@tecolab-global.com. We will advise on the quantity of sample required for testing.

You can deliver the sample to us via courier and provide us the tracking code. We will monitor the delivery status and inform you when we receive the sample.

Step 6: Track the status of your request

Once we have received your sample, we will provide a tracking number to you. You can easily track the status of your request here.

Step 7: Download your password-protected test report

Download your readily available test report online with just a single click using the link and password provided via email notification. We will receive a notification once you have downloaded the test report.

For safety purposes, the uploaded test report will self-destruct after 7 days.

Step 8: Analyze your passing criteria in the test report with our laboratory experts

The passing criteria are not specified within this test method as requirements and expectations will vary depending on regulatory agencies and the manufacturer’s requirements.

Need assistance with your test report? Discuss your results with our microbiologists for more information.