TEST METHODVAH Method 14.2

Surface Disinfection with Mechanical Action - 4-Field Test
(Fungicidal and Mycobactericidal Activity)
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VAH Method 14.2 is the VAH standard method used to evaluate the fungicidal and mycobactericidal activity of surface disinfectants with mechanical action through simulated-use carrier test, commonly known as 4-field test. The infographic below shows a brief insight of VAH 14.2.

As we know, there is currently no available EN standard method for surface disinfection with mechanical action for fungicidal and mycobactericidal activity. We developed VAH 14.2 in our lab so you can have your products tested against the full spectrum of activity without any gaps in your claims. Besides that, as a VAH approved lab, TECOLAB is also committed to providing the standards required for all your claims for VAH certification. If you are familiar with the EN 16615, VAH 14.2 is just the same 4-field test. Learn more below!

While we can perform all spectrum of activity according to this test method, our VAH 14.2 method is only accredited for fungicidal and mycobactericidal/tuberculocidal activity. For other activity claims, it is advisable to stick to EN 16615.

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CUSTOMER PROCESS
Step 1: Which category does your product fall under?

These are the categories of test products tested under VAH 14.2. This test method is advisable only for fungicidal and mycobactericidal/tuberculocidal activity.

  • Surface disinfectant applied by wiping
  • Ready-to-use wipes pre-soaked with surface disinfectant

If you are aiming for bactericidal and/or yeasticidal activity, we would suggest EN 16615.

If your product does not require a wiping action, please check out EN 17387 / EN 13697 (for bactericidal, yeasticidal and fungicidal activity) or VAH 14.1 (for mycobactericidal activity).

Step 2: Select your test microorganisms

These are the mandatory test organisms:

  • Mycobacterium terrae (for tuberculocidal activity)
  • Mycobacterium avium (additionally for mycobactericidal activity)
  • Aspergillus brasiliensis (for fungicidal activity)

Wish to add more microorganisms? Click here for our full list of microorganisms.

Step 3: Select an appropriate contact time and concentration for your test product

3 test concentrations is chosen according to the manufacturer’s recommendation, demonstrating at least 1 concentration in the active range and 1 in the non-active range.

2 contact times may be chosen from 1, 5, 15, 30, or 60 minutes depending on the claim and practicality of product use based on the manufacturer’s recommendation.

TEST RUN 1

1 carrier
2 contact times
3 concentrations

TEST RUN 2

2 carriers
Claimed concentration and contact time only

Step 4: What are the soiling conditions associated with your test product?

Interfering substances, or commonly referred to as soiling, are used to mimic organic substances found on human skin, surfaces, and instruments. These are the available soiling conditions according to the test method:

  • Clean condition: 0.3 g/L bovine serum albumin
  • Dirty condition: 3.0 g/L bovine serum albumin and 3.0 mL/L sheep erythrocytes
Step 5: Place an order and send your sample to us

Fill the test request form and place an order via email at info@tecolab-global.com. We will advise on the quantity of sample required for testing.

Check out our sample delivery service or you can deliver the sample to us via any courier and provide us the tracking code. We will monitor the delivery status and inform you when we receive the sample.

Step 6: Track the status of your request

Once we have received your sample, we will provide a tracking number to you. You can easily track the status of your request here.

Step 7: Download your password-protected test report

Download your readily available test report online with just a single click using the link and password provided via email notification. We will receive a notification once you have downloaded the test report.

For safety purposes, the uploaded test report will self-destruct after 7 days.

Step 8: Analyze your passing criteria in the test report with our laboratory experts

In order to pass the test, the product shall demonstrate at least a 4 decimal log (lg) reduction for both fungi and mycobacteria on test field 1. The mean number of colonies on test fields 2 to 4 shall not exceed 50.

Need assistance with your test report? Discuss your results with our microbiologists for more information.